Immunofluorescence of monoclonal antibody to PGM1 on HeLa cell. [antibody concentration 20 ug/ml]
PGM1 monoclonal antibody (M01), clone 3B8-H4 Western Blot analysis of PGM1 expression in Jurkat.
Western Blot analysis of PGM1 expression in transfected 293T cell line by PGM1 monoclonal antibody (M01), clone 3B8-H4.Lane 1: PGM1 transfected lysate(61.4 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of PGM1 transfected lysate using anti-PGM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM1 rabbit polyclonal antibody.
Detection limit for recombinant GST tagged PGM1 is 0.3 ng/ml as a capture antibody.
Western blot analysis of PGM1 over-expressed 293 cell line, cotransfected with PGM1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM1 monoclonal antibody (M01), clone 3B8-H4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.