Western blot analysis of extracts from various cell lines using PAX5 (D7H5X) XP ® Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower).
Immunohistochemical analysis of paraffin-embedded human tonsil using PAX5 (D7H5X) XP ® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using PAX5 (D7H5X) XP ® Rabbit mAb. Note staining of B cells.
Immunohistochemical analysis of paraffin-embedded cell pellets, Ramos (positive, left) or HeLa (negative, right), using PAX5 (D7H5X) XP ® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse spleen using PAX5 (D7H5X) XP ® Rabbit mAb.
Confocal immunofluorescent analysis of Ramos (left) and Jurkat (right) cells using PAX5 (D7H5X) XP ® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of human whole blood lymphocytes using PAX5 (D7H5X) XP ® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary Ab. The histogram overlay represents Pax5 signal intensity from gated CD19+ B lymphocytes (green) and CD3+ T lymphocytes (blue).