Western Blot analysis of PPBP expression in transfected 293T cell line by PPBP monoclonal antibody (M01), clone 3B9.Lane 1: PPBP transfected lysate(13.9 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged PPBP is approximately 0.03 ng/ml as a capture antibody.
Western blot analysis of PPBP over-expressed 293 cell line, cotransfected with PPBP Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PPBP monoclonal antibody (M01), clone 3B9 . GAPDH ( 36.1 kDa ) used as specificity and loading control.