Western blot analysis of extracts of HeLa cells, untreated or TPA-treated, using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated) and Streptavidin-HRP (#3999).
Immunoprecipitation of extracts from HeLa cells, untreated or TPA-treated, using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated) and Immobilized Streptavidin (Bead Conjugate) # 3419. Western blot analysis was performed using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb #9154, Mouse Anti-Rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 and Anti-mouse IgG, HRP-linked Antibody #7076 for detection.