Western Blot analysis of PPP3CB expression in transfected 293T cell line by PPP3CB monoclonal antibody (M01), clone 5D3.Lane 1: PPP3CB transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged PPP3CB is approximately 0.03 ng/ml as a capture antibody.
Western blot analysis of PPP3CB over-expressed 293 cell line, cotransfected with PPP3CB Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PPP3CB monoclonal antibody (M01), clone 5D3 . GAPDH ( 36.1 kDa ) used as specificity and loading control.