Parvalbumin alpha in Human Brain. Parvalbumin alpha was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 5 ug/ml Sheep Anti-Human/Mouse/Rat Parvalbumin alpha Antigen Affinity-purified Polyclonal Antibody overnight at 4°C. Tissue was stained with Anti-Sheep HRP-DAB (brown) and counterstained with hematoxylin (blue).
Detection of Human/Mouse/Rat Parvalbumin alpha by Western Blot. Western blot shows lysates of human, mouse, and rat brain tissue. PVDF membrane was probed with 1 ug/ml of Sheep Anti-Human/Mouse/Rat Parvalbumin alpha Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Parvalbumin alpha at approximately 12 kD (as indicated). This experiment was conducted under reducing conditions.