Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PHYH Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of PHYH (arrow) using rabbit polyclonal PHYH Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHYH gene.
Western blot of PHYH Antibody in MDA-MB435 cell line lysates (35 ug/lane). PHYH (arrow) was detected using the purified antibody.
PHYH Antibody flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.