Western blot of RFC5 (arrow) using rabbit polyclonal RFC5 Antibody (Center R185). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RFC5 gene.
Western blot of anti-RFC5 Antibody (Center R185) in rat PASMC cell line lysates (35 ug/lane). RFC5(arrow) was detected using the purified antibody( Provided by Hirotaka Ata).
Western blot of anti-RFC5 Antibody (Center R185) in HeLa cell line lysates (35 ug/lane). RFC5(arrow) was detected using the purified antibody.
RFC5 Antibody (Center R185) flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.