Detection of Human RNF25 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RNF25 antibody used for IP at 6 ug/mg lysate. RNF25 was also immunoprecipitated by rabbit anti-RNF25 antibody BL13794. For blotting immunoprecipitated RNF25, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human RNF25 by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (T; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RNF25 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.