Detection of Human rpL7a (SURF3) by Western Blot and Immunoprecipitation/Mass Spectrometry. Samples: A) Whole cell lysate from HeLa (20 and 4 ug), MCF-7 (7; 20 ug) and 293T (T; 20 ug) cells. B) Cytosolic and nuclear extract (10 mg/IP) from HeLa S3 cells. Antibodies: Affinity purified rabbit anti-rpL7a (SURF3) antibody used for WB at 0.1 ug/ml (A) and for IP at 0.7 ug/mg lysate (B). Detection: Chemiluminescence with an exposure time of 5 sec (A) and vMALDI-LTQ and LC-MS/MS of Commassie stained bands (B).