Western blot analysis of extracts from various cell lines using Brn2/POU3F2 (D2C1L) Rabbit mAb.
Confocal immunofluorescent analysis of P1 rat brain using Brn2 (D7H3) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Gated area shown at 10X magnification on right.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 A-375 cells and 10 μl of Brn2/POU3F2 (D2C1L) Rabbit mAb, using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared from 5 ng enriched ChIP DNA using NEBNext ® Ultra™ II DNA Library Prep Kit for Illumina ® , and sequenced on the Illumina NextSeq. The figure shows binding across the STRIP1 gene. For additional ChIP-seq tracks, please download the product data sheet.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 A-375 cells and either 10 μl of Brn2/POU3F2 (D2C1L) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human MITF Promoter Primers #12377 and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.