Western blot analysis of Recombinant PP1α, incubated with or without cdc2 protein kinase in the presence of ATP and kinase assay buffer at 30ºC for 30 minutes, using Phospho-PP1α (Thr320) Antibody (upper) or control PP1α Antibody #2582 (lower).
Western blot analysis of extracts from HeLa cells, asynchronous (lane 1), G2/M (lane 2) or released from G2/M for 6 hours (lane 3), using Phospho-PP1α (Thr320) Antibody (upper) or PP1α Antibody #2582 (lower).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using Phospho-PP1α (Thr320) Antibody.