Western blot analysis of extracts from A-431 cells, untreated or treated with EGF (50 ng/ml, 15 min), λ phosphatase (13333 Unit/ml, overnight) using Phospho-Prdx1 (Tyr194) (D1T9C) Rabbit mAb (upper) and Prdx1 (D5G12) Rabbit mAb #8499 (lower).
Immunoprecipitation of Phospho-Prdx1 (Tyr194) protein from A-431 cells treated with EGF (50 ng/ml, 15 min), using Rabbit (DA1E) mAb XP ® Isotype control #3900 (lane 2) or Phospho-Prdx1 (Tyr194) (D1T9C) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Prdx1 (Tyr194) (D1T9C) Rabbit mAb.