Syndecan-2 in Mouse Spinal Cord. Syndecan-2 was detected in perfusion fixed frozen sections of mouse spinal cord using Sheep Anti-Mouse Syndecan-2 Antigen Affinity-purified Polyclonal Antibody at 1.7 ug/ml overnight at 4°C. Tissue was stained using NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to the dorsal horn.
Detection of Mouse Syndecan-2 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, Balb/3T3 mouse embryonic fibroblast cell line, and B16-F1 mouse melanoma cell line. PVDF membrane was probed with 1 ug/ml of Sheep Anti-Mouse Syndecan-2 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Syndecan-2 at approximately 42 kD (as indicated). This experiment was conducted under reducing conditions.
Detection of Syndecan-2 in B16-F1 Mouse Cell Line by Flow Cytometry. B16-F1 mouse melanoma cell line was stained with Sheep Anti-Mouse Syndecan-2 Antigen Affinity-purified Polyclonal Antibody or control antibody, followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody.