Western blot analysis of extracts from various tissues and cell lines using PRNP (D3Q5C) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of PRNP from SK-MEL-28 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or PRNP (D3Q5C) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PRNP (D3Q5C) Rabbit mAb.
Confocal immunofluorescent analysis of SK-MEL-28 (positive, left) or MCF7 (negative, right) cells, using PRNP (D3Q5C) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).