Progesterone Receptor A/B (D8Q2J) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 8757
• Prices: $109.00, $277.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D8Q2J
• Applications: WB IP IHC-P ICC/IF FC ChIP
• Species Reactivities: Human, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr541 of human progesterone receptor protein.
• Description: Rabbit Monoclonal
• Gene: PGR

Product images

Western blot analysis of extracts from T-47D (PR positive) and MDA-MB-231 (PR negative) cells using Progesterone Receptor A/B (D8Q2J) XP ® Rabbit mAb (upper) or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower).

Western blot analysis of extracts from T-47D cells, grown for 48 hr in phenol red-free medium supplemented with 5% charcoal-stripped FBS and then treated with either a vehicle control (-) or promegestone (R5020, 100 nM, 16 hr; +), using Progesterone Receptor A/B (D8Q2J) XP ® Rabbit mAb (upper) or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). Prolonged treatment of PR-expressing cells with R5020 is known to induce PR downregulation and hyperphosphorylation, which is reflected by slower migration on SDS-PAGE.

Immunohistochemical analysis of paraffin-embedded human infiltrating ductal breast carcinoma using Progesterone Receptor A/B (D8Q2J) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded cell pellets, T-47D (high PR, left), MCF-7 (low PR, middle) and MDA-MB-231 (PR negative, right), using Progesterone Receptor A/B (D8Q2J) XP ® Rabbit mAb.

Confocal immunofluorescent analysis of T-47D (PR positive, left) and MDA-MB-231 (PR negative, right) cells using Progesterone Receptor A/B (D8Q2J) XP ® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Flow cytometric analysis of MDA MB-231 cells (blue) and T47D cells using Progesterone REceptor A/B (D8Q2J) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (ALexa FLuor® 488 Conjugate) #4412 was used as a secondary antibody.

T-47D cells were cultured in phenol red-free media supplemented with 5% charcoal-stripped FBS for 48 hr and then either untreated (left panel) or promegestone-treated (R5020, 10 nM, 1 hr; right panel). Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 cells and 5 µl of Progesterone Receptor A/B (D8Q2J) XP ® Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human FKBP51 Intron 5 Primers #7859, human E2F-1 proximal enhancer site #1 primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.