Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human PSMA1 (hPSMA1-Myc/DDK; +), full-length human PSMA2 (hPSMA2-Myc/DDK; +), full-length human PSMA3 (hPSMA3-Myc/DDK; +), full-length human PSMA4 (hPSMA4-Myc/DDK; +), full-length human PSMA6 (hPSMA6-Myc/DDK; +), or full-length human PSMA7 (hPSMA7-Myc/DDK; +), using PSMA3 (D4Y9O) Rabbit mAb (upper) or DYKDDDDK Tag Antibody #2368 (lower).
Immunoprecipitation of PSMA3 from 293T cell extracts, using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or PSMA3 (D4Y9O) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PSMA3 (D4Y9O) Rabbit mAb.