PSMB8/LMP7 (1A5) Mouse mAb

• Supplier: Cell Signaling Technology
• Catalog: 13726
• Prices: $246.00
• Sizes: 100 µl (10 western blots)
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 1A5
• Applications: WB ICC/IF FC
• Species Reactivities: Human, Rat
• Antigen: Monoclonal antibody is produced by immunizing animals with recombinant protein encompassing the full-length of human PSMB8/LMP7 protein.
• Description: Mouse Monoclonal
• Gene: PSMB8

Product images

Western blot analysis of extracts from various cell lines using PSMB8/LMP7 (1A5) Mouse mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). (The T2 (13) and RKO (8) cell lines are both deficient in PSMB8/LMP8 protein expression).

Western blot analysis of extracts from HeLa and SW620 cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml, 72 hr; +), using PSMB8/LMP7 (1A5) Mouse mAb (upper) and GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower).

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human PSMB5 (hPSMB5-Myc/DDK; +) or Myc/DDK-tagged full-length human PSMB8 (hPSMB8-Myc/DDK; +), using PSMB8/LMP7 (1A5) Mouse mAb (upper) and DYKDDDDK Tag Antibody #2368 (lower).

Confocal immunofluorescent analysis of HeLa cells, untreated (upper, left) or treated with hIFN-γ #8901 (100 ng/ml, 72 hr; upper, right), or ACHN cells (lower, left) and RKO cells (lower, right), using PSMB8/LMP7 (1A5) Mouse mAb (green) and β-Actin (13E5) Rabbit mAb #4970 (red).

Flow cytometric analysis of Raji cells using PSMB8/LMP7 (1A5) Mouse mAb (blue) compared to Mouse (G3A1) mAb IgG1 Isotype Control #5415 (Red). Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4408 was used as a secondary antibody.