Western blot analysis of MYPT1 phosphorylation in untreated (A,D), Calyculin A treated (B,E) and Calyculin A and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-MYPT1 (F-11): sc-377531 (A,B,C) and MYPT1 (H-130): sc-25618 (D,E,F).
Western blot analysis of MYPT1 phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) HeLa whole cell lysates. Antibodies tested include p-MYPT1 (F-11): sc-377531 (A,B) and MYPT1 (H-130): sc-25618 (C,D).
Western blot analysis of MYPT1 phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) HCT-8 whole cell lysates. Antibodies tested include p-MYPT1 (F-11): sc-377531 (A,B) and MYPT1 (H-130): sc-25618 (C,D).