Detection of Human MCT8 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T and cells. Antibodies: Affinity purified rabbit anti-MCT8 antibody used for IP at 6 ug per reaction. MCT8 was also immunoprecipitated by rabbit anti-MCT8 antibody BL16310. For blotting immunoprecipitated MCT8, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human and Mouse MCT8 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-MCT8 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.