Formalin-fixed and paraffin-embedded human colon carcinoma reacted with ARGBP2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Fluorescent image of U251 cell stained with ARGBP2 Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARGBP2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). ARGBP2 immunoreactivity is localized to Nucleus significantly.
Western blot of ARGBP2 Antibody in MDA-MB231 cell line lysates (35 ug/lane). ARGBP2 (arrow) was detected using the purified antibody.
Flow cytometric of MDA-231 cells using ARGBP2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.