Detection of Human Sp1 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Sp1 used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB.
Detection of Mouse Sp1 by Immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Sp1 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Detection of Human Sp1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. Antibodies: Affinity purified goat anti-Sp1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Mouse Sp1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, Renca, and TCMK-1 cells. Antibodies: Affinity purified goat anti-Sp1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.