Confocal immunofluorescent analysis of C2C12 cells, treated with LY294002 #9901 (left) or insulin (right), using Phospho-NDRG1 (Thr346) (D98G11) XP ® Rabbit mAb (Alexa Fluor ® 594 Conjugate) (red). Actin filaments were labeled with Alexa Fluor ® 488 phalloidin (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).
Confocal immunofluorescent analysis of 786-O (high-expressing, left), MDA-MB-435 (low-expressing, middle), and A-204 (low-expressing, right) cells using Phospho-NDRG1 (Thr346) (D98G11) XP ® Rabbit mAb (Alexa Fluor ® 594 Conjugate) (red). Actin filaments were labeled with Alexa Fluor ® 488 phalloidin (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).