SPARC-like 1/SPARCL1 in Human Brain. SPARC-like 1/SPARCL1 was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human SPARC-like 1/SPARCL1 Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4°C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons.
Detection of Human SPARC-like 1/SPARCL1 by Western Blot. Western blot shows lysates of human lung tissue. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human SPARC-like 1/ SPARCL1 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for SPARC-like 1/SPARCL1 at approximately 130 kD (as indicated). This experiment was conducted under reducing conditions.
Detectionof SPARClike1/SPARCL1 in HL60 Human Cell Line by Flow Cytometry. HL60 human acute promyelocytic leukemia cell line was stained with Goat Anti-Human SPARC-like 1/SPARCL1 Antigen Affinity-purified Polyclonal Antibody or control antibody, followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.