SPHK1 Antibody (C-term R414)IHC of formalin-fixed and paraffin-embedded skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of the SPHK1 Antibody (C-term R414) for immunohistochemistry.
Western blot of SPHK1 (arrow) using rabbit polyclonal SPHK1 Antibody (C-term R414). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene.
Western blot of SPHK1 Antibody (C-term R414) in 293 cell line lysates (35 ug/lane). SPHK1 (arrow) was detected using the purified antibody.
SPHK1 Antibody (C-term R414) flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.