Detection of Human SRP68 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SRP68 antibody used for IP at 6 ug/mg lysate. SRP68 was also immunoprecipitated by rabbit anti-SRP68 antibody BL14251. For blotting immunoprecipitated SRP68, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of Mouse SRP68 by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, Renca, and NIH3T3 cells. Antibodies: Affinity purified rabbit anti-SRP68 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human SRP68 by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), and Jurkat (50 ug) cells. Antibodies: Affinity purified rabbit anti-SRP68 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.