Western Blot analysis of STAR expression in transfected 293T cell line by STAR monoclonal antibody (M01), clone 5F9.Lane 1: STAR transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged STAR is approximately 0.03 ng/ml as a capture antibody.
Western blot analysis of STAR over-expressed 293 cell line, cotransfected with STAR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with STAR monoclonal antibody (M01), clone 5F9 . GAPDH ( 36.1 kDa ) used as specificity and loading control.