Western blot analysis of extracts from HeLa cells, untreated or treated with CIP and λ phosphatase, using Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb (upper) or Rad17 (D3G6) Rabbit mAb #8561 (lower).
Western blot analysis of extracts from MRC-5 cells, untreated or UV-treated (100 mJ/cm 2 , 2 hr recovery), using Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb.
Immunoprecipitation of phosphorylated Rad17 from MRC-5 cells, untreated or UV-treated (100 mJ/cm 2 , 2 hr recovery), using Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb. Western blot was performed using the same antibody. Lanes 1 and 3 are 10% input.