Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
SPAK Antibody (A363) western blot of U937 cell line lysates (35 ug/lane). The SPAK antibody detected the SPAK protein (arrow).
Western blot of SPAK (arrow) using rabbit polyclonal SPAK Antibody (A363). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPAK gene.
Western blot of SPAK (arrow) using rabbit polyclonal SPAK Antibody (A363). 293T cell lysates either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPAK gene.
Western blot of lysate from HepG2 cell line, using SPAK Antibody (A363). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Western blot of lysate from 293 cell line, using SPAK Antibody (A363). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot of anti-SPAK antibody in mouse liver tissue lysate. SPAK (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.