![Anti-Swi6 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S. pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of ~43 kD corresponding to S. pombe Swi6 protein. ~35ug of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 um nitrocellulose. The blot was incubated with a 1:1700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://www.bioprodhub.com/system/product_images/ab_products/3/sub_5/24870_62897_1327161.jpg)
Anti-Swi6 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S. pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of ~43 kD corresponding to S. pombe Swi6 protein. ~35ug of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 um nitrocellulose. The blot was incubated with a 1:1700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Schematic model of S. pombe centromere cluster in interphase cells. The distribution of Ndc80, Cnp1, and Swi6 proteins are indicated in relation to the observed centromeric anchor and heterochromatin structures. See Kniola et al for additional details.