Detection of Human Synaptogyrin-1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from 293T and cells. Antibodies: Affinity purified rabbit anti-Synaptogyrin-1 antibody used for IP at 6 ug per reaction. Synaptogyrin-1 was also immunoprecipitated by rabbit anti-Synaptogyrin-1 antibody BL16699. For blotting immunoprecipitated Synaptogyrin-1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human and Mouse Synaptogyrin-1 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-Synaptogyrin-1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.