Anti-TBX1 / Brachyury antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10717 dilution 1:75.
Fluorescent confocal image of A2058 cell stained with TBX1 Antibody. A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TBX1 immunoreactivity is localized to nucleus significantly.
Western blot of lysate from HLVEC cell line, using TBX1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
TBX1 Antibody western blot of HLVEC cell line lysates (35 ug/lane). The TBX1 antibody detected the TBX1 protein (arrow).