RCAS1 (D2B6N) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 12290
• Prices: $109.00, $277.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D2B6N
• Applications: WB IP ICC/IF FC
• Species Reactivities: Human, Mouse, Rat
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly147 of human RCAS1 protein.
• Description: Rabbit Monoclonal
• Gene: EBAG9

Product images

Western blot analysis of extracts from various cell lines using RCAS1 (D2B6N) XP ® Rabbit mAb.

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human RCAS1 (hRCAS1-Myc/DDK; +), using RCAS1 (D2B6N) XP ® Rabbit mAb.

Immunoprecipitation of RCAS1 from HCT 116 extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or RCAS1 (D2B6N) XP ® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using RCAS1 (D2B6N) XP ® Rabbit mAb.

Confocal immunofluorescent analysis of MCF7 cells, untreated (left) or treated with Brefeldin A #9972 (5 μg/ml, 1 hr; right), using RCAS1 (D2B6N) XP ® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Flow cytometric analysis of Jurkat cells using RCAS1 (D2B6N) XP ® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (red).