Western blot analysis of extracts from Jurkat cells, untreated (-) or calf intestinal phosphatase (CIP)-treated (+), using Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb (upper) or REPS1 (D6F4) Rabbit mAb #6404 (lower).
Immunoprecipitation of phospho-REPS1 (Ser709) from Jurkat cell extracts, using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb.