
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TOE1 mAb.

Western blot analysis of immunized recombinant protein, using anti-TOE1 monoclonal antibody.

Detection of human TOE1 by Western blot. Samples: Whole cell lysate (25 ug) from HeLa cells. Predicted molecular weight: 56 kDa

Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-TOE1 mAb. Predicted molecular weight: 56 kDa