Mouse cerebellar cortex showing molecular cell layer (mc), Purkinje cells (Pc) and granular cell layer. Trxl-1 antibody gives strong nuclear labeling in Purkinje cells and granular cells with lower cytoplasmic staining. The results correspond precisely to TrxL-1 mRNA expression shown with in situ hybridization (Jimenez A et al. FEBS Lett. 2006, 580(3):960-7) and could be abolished with peptide used for immunization. The fixation was 4% paraformaldehyde perfusion (4mins) and 60 mins immersion. Staining was done in frozen sections with Vector Elite kit and nickel intensified DAB as a chromogen. Data courtesy of Prof. Markku Pelto-Huikko, Department of Developmental Biology, Medical School,Tampere University, Finland.
The anti-TrxL antibody is used in Western blot to detect TrxL in HL-60 cell lysate.