Detection of Human TTLL4 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-TTLL4 antibody used for IP at 6 ug/mg lysate. TTLL4 was also immunoprecipitated by rabbit anti-TTLL4 antibody BL15510. For blotting immunoprecipitated TTLL4, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human TTLL4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-TTLL4 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.