Anti-UBC13 Antibody - Western Blot. Western blot of affinity purified anti-UBC13 antibody shows detection of UBC13 protein in human small intestine lysate (lane 1) but not in mouse thymus lysate (lane 2). The heavily stained band in lane 1 (arrowhead) indicates this particular gel was overloaded with protein. The identity of minor reactive bands is unknown but could represent E2 complexes. Each lane contains approximately 20 ug of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:10000 dilution of Alexa FluorTM 680 conjugated Rb-a-Goat IgG. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Other detection systems will yield similar results.