Detection of Mouse UBXD7 by Western Blot. Samples: Whole cell lysate (50 ug) from mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-UBXD7 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human UBXD7 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-UBXD7 antibody used for IP at 6 ug/mg lysate. UBXD7 was also immunoprecipitated by rabbit anti-UBXD7 antibody BL13458. For blotting immunoprecipitated UBXD7, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of Human UBXD7 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-UBXD7 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.