Anti-UCHL1 / PGP9.5 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Fluorescent image of U251 cell stained with UCHL1 Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with UCHL1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). UCHL1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
Western blot of lysates from SH-SY5Y cell line and mouse brain tissue (from left to right),using UCHL1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
UCHL1 Antibody western blot of U266,NCI-H1299,mouse Neuro-2a cell line and mouse brain tissue lysates (35 ug/lane). The UCHL1 antibody detected the UCHL1 protein (arrow).