Sec24B (D7D6S) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 12042
• Prices: $246.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D7D6S
• Applications: WB IP ICC/IF
• Species Reactivities: Human, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr270 of human Sec24B protein.
• Description: Rabbit Monoclonal
• Gene: SEC24B

Product images

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® Sec24B siRNA I #12229 (+), or SignalSilence ® Sec24B siRNA II #12333 (+), using Sec24B Antibody #7427 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The Sec24B Antibody confirms silencing of Sec24B expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.

Western blot analysis of extracts from various cell lines using Sec24B (D7D6S) Rabbit mAb.

Immunoprecipitation of Sec24B from HeLa cell extracts, using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Sec24B (D7D6S) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Sec24B (D7D6S) Rabbit mAb.

Confocal immunofluorescent analysis of HeLa cells, transfected with Signal Silence ® Control siRNA (Unconjugated) #6568 (left), SignalSilence ® Sec24B siRNA I #12229 (center), or SignalSilence ® Sec24B siRNA II #12333 (right), using Sec24B (D7D6S) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).