XRCC6 Antibody IHC of formalin-fixed and paraffin-embedded human lung carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
Confocal immunofluorescent of XRCC6 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Fluorescent confocal image of HeLa cell stained with XRCC6 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with XRCC6 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). XRCC6 immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.
Western blot of XRCC6 Antibody in A2058 cell line lysates (35 ug/lane). XRCC6 (arrow) was detected using the purified antibody.
Western blot of XRCC6 (arrow) using rabbit polyclonal XRCC6 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the XRCC6 gene.
XRCC6 Antibody flow cytometry of A2058 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.