ZNF202 Antibody IHC of formalin-fixed and paraffin-embedded breast carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
Confocal immunofluorescent of ZNF202 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Fluorescent confocal image of MDA-MB231 cell stained with ZNF202 Antibody. MDA-MB231 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZNF202 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ZNF202 immunoreactivity is localized to nucleus significantly.
Western blot of ZNF202 Antibody in HepG2 cell line lysates (35 ug/lane). ZNF202 (arrow) was detected using the purified antibody.
ZNF202 Antibody flow cytometry of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.