ATG5 Antibody

• Supplier: Novus Biologicals
• Catalog: NB110-53818
• Prices: $149.00, $349.00
• Sizes: 25 µl, 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB Simple Western ICC/IF IHC IHC-P IP PLA
• Species Reactivities: Human, Rat, Bovine, Zebrafish
• Antigen: A synthetic peptide made to an N-terminal region of the human ATG5 protein (within residues 1-50) [Swiss-Prot Q9H1Y0]
• Purity/Format: Immunogen affinity purified
• Blocking Peptide: ATG5 Blocking Peptide
• Description: Rabbit Polyclonal
• Gene: ATG5
• Conjugate: Unconjugated

Product images

Western Blot: ATG5 Antibody [NB110-53818] - Total protein from Human HeLa and A431 and Mouse MEF cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ATG5 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NB110-53818] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ATG5 [NB110-53818] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: ATG5 Antibody [NB110-53818] - Staining of human liver hepatocytes at 2.5ug/ml. 40X magnification.

Western Blot: ATG5 Antibody [NB110-53818] - Analysis in mouse wildtype ES cell lysate (Lane 1) using NB110-53818. Lane 2 is a mouse ATG5 KO ES cell lysate (negative control). Atg5-/- ES cells from Dr. Noboru Mizushima [Mizushima, N. et al. J. Cell Biol. 152 (2001)] Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center.

Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NB110-53818] - Staining of SY5Y cells at 1:250. Incubated overnight at 4 degrees. Photo courtesy of an anonymous collaborator.

Immunohistochemistry: ATG5 Antibody [NB110-53818] - Staining mouse intestine using DAB with hematoxylin counterstain.

Immunohistochemistry-Paraffin: ATG5 Antibody [NB110-53818] - Staining of mouse knee. Image from verified customer review.

Simple Western: ATG5 Antibody [NB110-53818] - Simple Western lane view shows a specific band for ATG5 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.