CHREBP Antibody

• Supplier: Novus Biologicals
• Catalog: NB400-135
• Prices: $129.00, $349.00
• Sizes: 25 µl, 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB ChIP IB ICC/IF IHC IHC-P IP
• Species Reactivities: Human, Rat
• Antigen: A C-terminal synthetic peptide made to the human CHREBP protein sequence (between residues 800-852). [UniProt# Q9NP71, Isoform 1/Alpha]
• Purity/Format: Immunogen affinity purified
• Blocking Peptide: CHREBP Blocking Peptide
• Description: Rabbit Polyclonal
• Gene: MLXIPL
• Conjugate: Unconjugated

Product images

Western Blot: CHREBP Antibody [NB400-135] - Detection of ChREBP in liver nuclear extracts from well-fed rats. 7% SDS gel, 1:1,000 dilution of NB400-135. Photo courtesy of Dr. Uyeda, UT Southwestern University.

Immunocytochemistry/Immunofluorescence: CHREBP Antibody [NB400-135] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ChREBP (NB400-135) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: CHREBP Antibody [NB400-135] - Colon, Epithelium 40X.

Western Blot: CHREBP Antibody [NB400-135] - Detection of ChREBP in 20 ug of human hepatocyte lysate using NB400-135. 5-10 second film exposure.

Immunocytochemistry/Immunofluorescence: CHREBP Antibody [NB400-135] - Immunofluorescent staining of a human hepatocyte using NB400-135. Overlay staining of ChREBP (rhodamine red) and DAPI nuclear staining (blue).

Immunohistochemistry: CHREBP Antibody [NB400-135] - Analysis of CHREBP in mouse liver using DAB with hematoxylin counterstain.