SOX2 Antibody

• Supplier: Novus Biologicals
• Catalog: NB110-37235
• Prices: $129.00, $349.00
• Sizes: 25 µl, 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB FC ICC/IF IHC IHC-P
• Species Reactivities: Human, Sheep, Chimpanzee
• Antigen: A synthetic peptide made to the N-terminal region of human SOX2 protein (within residues 1-100). [Swiss-Prot# P48431]
• Purity/Format: Immunogen affinity purified
• Blocking Peptide: SOX2 Blocking Peptide
• Description: Rabbit Polyclonal
• Gene: SOX2
• Conjugate: Unconjugated

Product images

Western Blot: SOX2 Antibody [NB110-37235] - Detection of SOX2 in mouse brain lysate using NB110-37235 (0.5ug/ml).

Immunocytochemistry/Immunofluorescence: SOX2 Antibody [NB110-37235] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-SOX2 (NB110-37235) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: SOX2 Antibody [NB110-37235] - Staining of human uterus, endometrial glands.

Flow Cytometry: SOX2 Antibody [NB110-37235] - Staining of NTERA-2 cells using NB110-37235 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit IgG secondary antibody.

Immunocytochemistry/Immunofluorescence: SOX2 Antibody [NB110-37235] - ICC/IF staining of SOX2 in NTERA2 cells with Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained with Dylight 550 (Red) and DAPI (Blue), respectively.