Histone H3 [Trimethyl Lys4] Antibody

• Supplier: Novus Biologicals
• Catalog: NB21-1023
• Prices: $199.00, $349.00
• Sizes: 25 µg, 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB ChIP DB ICC/IF
• Species Reactivities: Human, C. elegans, Rat, Chimpanzee, Plant
• Antigen: Synthetic trimethylated peptide surrounding Lysine 4 of human Histone H3.2 [Swiss Prot Q71DI3].
• Purity/Format: Immunogen affinity purified
• Blocking Peptide: Histone H3 [Trimethyl Lys4] Blocking Peptide
• Description: Rabbit Polyclonal
• Gene: HIST2H3C
• Conjugate: Unconjugated

Product images

Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - Western blot analysis of HeLa histone prep (A), NIH 3T3 histone prep (B), HeLa whole cell extract (C), and NIH 3T3 whole cell extract (D) using Histone H3 [Trimethyl Lys4] antibody (NB21-1023) at 1 ug/ml.

Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - Histone H3 K4me3 antibody tested in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).

Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1023 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.

Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - WB analysis of Histone H3 K4me3 in C. elegans embryo nuclear extract.

Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - WB analysis of Histone H3 K4me3 in 1. HeLa histone prep and 2. NIH 3T3 histone prep lysates.

Dot Blot: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - Dot Blot analysis of Histone H3 K4me3 using the peptides listed above in 10, 3, and 1 picomoles of peptide.

Histone H3 [Trimethyl Lys4] Antibody [NB21-1023]