![Western Blot: Perilipin-2/ADFP Antibody [NB110-40877] - Detection of murine ADFP in mouse liver lysate.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_6/28281_Perilipin-2-ADFP-Antibody-Western-Blot-NB110-40877-img0005.jpg)
Western Blot: Perilipin-2/ADFP Antibody [NB110-40877] - Detection of murine ADFP in mouse liver lysate.
![Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody [NB110-40877] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Perilipin-2/ADFP at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_6/28282_Perilipin-2-ADFP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40877-img0008.jpg)
Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody [NB110-40877] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Perilipin-2/ADFP at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
![Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody [NB110-40877] - Detection of ADFP (Green) in Hela cells using NB110-40877 at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_6/28283_Perilipin-2-ADFP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40877-img0004.jpg)
Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody [NB110-40877] - Detection of ADFP (Green) in Hela cells using NB110-40877 at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.
![Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody [NB110-40877] - ADFP antibody was tested in mouse liver using DAB with hematoxylin counterstain.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_6/28284_Perilipin-2-ADFP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40877-img0006.jpg)
Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody [NB110-40877] - ADFP antibody was tested in mouse liver using DAB with hematoxylin counterstain.
![Simple Western: Perilipin-2/ADFP Antibody [NB110-40877] - Simple Western lane view shows a specific band for ADFP in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_6/28285_Perilipin-2-ADFP-Antibody-Simple-Western-NB110-40877-img0007.jpg)
Simple Western: Perilipin-2/ADFP Antibody [NB110-40877] - Simple Western lane view shows a specific band for ADFP in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.