Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody [NB300-129] - Immunofluorescent staining of aggresomes of mouse L-cells treated with lactacysteine, using NB300-129.
Immunohistochemistry-Paraffin: Ubiquitin Antibody [NB300-129] - IHC staining of Ubiquitin in human rectal cancer using DAB with hematoxylin counterstain.
Ubiquitin Antibody [NB300-129] - Western blot analysis of Hek 293 cell lysates using Rabbit pAb to ubiquitin (1:5000 dilution, green) and Mouse mAb to B-Tubulin (1:10000 dilution, red) used as a loading control. Cells were maintained in normal medium (Control-Ct) or treated with proteasome inhibitor lactacystin (Lc) at 10 um for 16 h. Lysed cells were electrophoresed on 4-20% SDS-PAGE, and blotted to PVDF membrane. Note the smeary-patterned reactivities detected above the 200kDa standard that presumably represent accumulation of ubiquitinated proteins in proteasome inhibitor-Lc treated cells. Prominent band at 8kDa corresponds to monoubiquitin