![Western Blot: TLR4 Antibody [NBP2-24821] - Analysis of TLR4 using TLR4 antibody at 2 ug/ml on partial recombinant mouse TLR4 protein.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_6/29107_TLR4-Antibody-Western-Blot-NBP2-24821-img0001.jpg)
Western Blot: TLR4 Antibody [NBP2-24821] - Analysis of TLR4 using TLR4 antibody at 2 ug/ml on partial recombinant mouse TLR4 protein.
![Immunocytochemistry/Immunofluorescence: TLR4 Antibody [NBP2-24821] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-TLR4 [NBP2-24821] at a 1:50 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_6/29108_TLR4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24821-img0006.jpg)
Immunocytochemistry/Immunofluorescence: TLR4 Antibody [NBP2-24821] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-TLR4 [NBP2-24821] at a 1:50 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
![Immunohistochemistry-Paraffin: TLR4 Antibody [NBP2-24821] - Analysis of TLR4 in formalin-fixed, paraffin-embedded normal (A) and tumor (B) prostate sections using TLR4 antibody at 1:50 dilution. ICC analysis of TLR4 in formalin-fixed Ramos (C) and 293 (D) cells using antibody at 1:10 dilution. Data courtesy of Dr. Regina Gandour-Edwards](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_6/29109_TLR4-Antibody-Immunohistochemistry-Paraffin-NBP2-24821-img0002.jpg)
Immunohistochemistry-Paraffin: TLR4 Antibody [NBP2-24821] - Analysis of TLR4 in formalin-fixed, paraffin-embedded normal (A) and tumor (B) prostate sections using TLR4 antibody at 1:50 dilution. ICC analysis of TLR4 in formalin-fixed Ramos (C) and 293 (D) cells using antibody at 1:10 dilution. Data courtesy of Dr. Regina Gandour-Edwards
![Flow Cytometry: TLR4 Antibody [NBP2-24821] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-24821. Staining of RAW cells were probed using 2 ug of TLR4 antibody (red) and 2 ug of isotype control antibody (20304AF488, green). Novus's TLR cell surface flow kit (10099K) was used for this test.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_6/29110_TLR4%2520Antibody-Flow%2520Cytometry-NBP2-24821-img0005.jpg)
Flow Cytometry: TLR4 Antibody [NBP2-24821] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-24821. Staining of RAW cells were probed using 2 ug of TLR4 antibody (red) and 2 ug of isotype control antibody (20304AF488, green). Novus's TLR cell surface flow kit (10099K) was used for this test.
![Flow Cytometry: TLR4 Antibody [NBP2-24821] - Cell surface analysis using TLR4 antibody. Mouse macrophage cells were probed using 1 ug of TLR4 antibody (red) and 1 ug of isotype control antibody (green). This antibody and PE-conjugated secondary were used for this test.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_6/29111_TLR4-Antibody-Flow-Cytometry-NBP2-24821-img0003.jpg)
Flow Cytometry: TLR4 Antibody [NBP2-24821] - Cell surface analysis using TLR4 antibody. Mouse macrophage cells were probed using 1 ug of TLR4 antibody (red) and 1 ug of isotype control antibody (green). This antibody and PE-conjugated secondary were used for this test.